A kinetic model of the central carbon metabolism for acrylic acid production in Escherichia coli

Acrylic acid is a value-added chemical used in industry to produce diapers, coatings, paints, and adhesives, among many others. Due its economic importance, there currently need for new sustainable ways synthesise it. Recently, the focus has been laid use of Escherichia coli express full bio-based pathway using 3-hydroxypropionate as an intermediary through three distinct pathways (glycerol, malonyl-CoA, ? -alanine). Hence, goals this work were COPASI software assess which higher potential industrial-scale production, from either glucose or glycerol, identify targets improve biosynthetic yields. When compared available literature, models developed during successfully predict production 3-hydroxypropionate, glycerol carbon source pathway, malonyl-CoA -alanine pathways. Finally, allowed four over-expression (glycerol-3-phosphate dehydrogenase (G3pD), acetyl-CoA carboxylase (AccC), aspartate aminotransferase (AspAT), (AspC)) that should, theoretically, result AA